Expression patterns of regulatory RNAs and proteins
Bioinformatics approach to identify interactions between regulatory RNAs and target RNAs & novel experimental target search strategies for miRNA and other small RNAs.
Structure and conformational changes of regulatory RNAs
In vitro studies (chemical probing, chemical interference, mutagenesis, kinetic binding experiments, microcalorimetry) & siRNA strategies for phenotypic knock-downs.
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Biological roles of small RNAs
Knocked out or over-expressed strains, heterologous expression of regulatory RNAs and their targets to explore regulatory consequences on cellular processes and phylogenetic relationship.
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